85-4776-76　［取扱停止］PAF (Platelet Activating Factor) BioAssay™ ELISA Kit (Human) 96Tests　396459

特徴

• Sample Type:Serum, plasma and other biological fluids.
• Intended Use:For quantitative detection of PAF in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.
• Sensitivity:<0.938ng/ml
• Precision:Intra-Assay CV: <8%Inter-Assay CV: <10%
• Range:1.563-100ng/ml
• Specificity:Human
• Test Principle:This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this BioAssay™ kit has been pre-coated with PAF . During the reaction, PAF in the sample or standard competes with a fixed amount of PAF on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to PAF. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of PAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Kit Components:Microtiter Strips, 1x96 wells (8x12 wells)Standard, 2x1 vialSample/Standard Dilution Buffer, 1x20mlAntibody (Biotin) (Concentrated), 1x120ulAntibody Dilution Buffer, 1x10mlStreptavidin (HRP) (SABC), 1x120ulSABC Dilution Buffer, 1x10mlTMB Substrate, 1x10mlStop Solution, 1x10mlWash Buffer, 25X, 1x30ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.