85-4776-45　［取扱停止］LZMc (Lysozyme C) BioAssay™ ELISA Kit (Human) 48Tests　396444

特徴

• Sample Type:Serum, plasma and other biological fluids.
• Intended Use:For quantitative detection of LZM C in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.
• Sensitivity:<5.625ng/ml
• Precision:Intra-Assay CV: <8%Inter-Assay CV: <10%
• Range:9.375-600ng/ml
• Specificity:Human
• Test Principle:This BioAssay™ kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with LZM C. During the reaction, LZM C in the sample or standard competes with a fixed amount of LZM C on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to LZM C. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of LZM C in the samples is then determined by comparing the OD of the samples to the standard curve.
• Kit Components:Microtiter Strips, 1x96 wells (8x12 wells)Standard, 2x1 vialSample/Standard Dilution Buffer, 1x20mlAntibody (Biotin) (Concentrated), 1x120ulAntibody Dilution Buffer, 1x10mlStreptavidin (HRP) (SABC), 1x120ulSABC Dilution Buffer, 1x10mlTMB Substrate, 1x10mlStop Solution, 1x10mlWash Buffer, 25X, 1x30ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.