85-4776-40　［取扱停止］L-FABP (Liver Fatty Acid Binding Protein) BioAssay™ ELISA Kit (Bovine) 96Tests　396438

特徴

• Sample Type:Serum, plasma, tissue homogenates and other biological fluids.
• Intended Use:For quantitative detection of L-FABP in serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity:<0.469ng/ml
• Precision:Intra-Assay CV: <8%Inter-Assay CV: <10%
• Range:0.781-50ng/ml
• Specificity:Bovine
• Test Principle:This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-L-FABP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-L-FABP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the L-FABP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of L-FABP can be calculated.
• Kit Components:Microtiter Strips, 1x96 wells (8x12 wells)Standard, 2x1 vialSample/Standard Dilution Buffer, 1x20mlAntibody (Biotin) (Concentrated), 1x120ulAntibody Dilution Buffer, 1x10mlStreptavidin (HRP) (SABC), 1x120ulSABC Dilution Buffer, 1x10mlTMB Substrate, 1x10mlStop Solution, 1x10mlWash Buffer, 25X, 1x30ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.