85-4776-01　［取扱停止］HSPBP1 (Hsp70-Binding Protein 1) BioAssay™ ELISA Kit (Human) 48Tests　396416

特徴

• Sample Type:Serum, plasma and other biological fluids.
• Intended Use:For quantitative detection of HSPBP1 in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.
• Sensitivity:<0.188ng/ml
• Precision:Intra-Assay CV: <8%Inter-Assay CV: <10%
• Range:0.313-20ng/ml
• Specificity:Human
• Test Principle:This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HSPBP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HSPBP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HSPBP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HSPBP1 can be calculated.
• Kit Components:Microtiter Strips, 1x96 wells (8x12 wells)Standard, 2x1 vialSample/Standard Dilution Buffer, 1x20mlAntibody (Biotin) (Concentrated), 1x120ulAntibody Dilution Buffer, 1x10mlStreptavidin (HRP) (SABC), 1x120ulSABC Dilution Buffer, 1x10mlTMB Substrate, 1x10mlStop Solution, 1x10mlWash Buffer, 25X, 1x30ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.