85-4775-79　Hepatitis B Virus Surface Antibody (HBsAb) BioAssay™ Qualitative ELISA Kit (Human) 96Tests　396401

特徴

• The HBsAb ELISA Kit allows for the in vitro qualitative determination of HBsAb human serum and plasma.
• Precision:Intra-Assay CV: <10%Inter-Assay CV: <10%
• Assay Principle:This kit is based on sandwich enzyme-linked immunosorbent assay technology. HBsAg is pre-coated onto the 96-well plates. The test samples and HBsAg (HRP) are added to the wells and if there is any HBsAb in the samples, it will form a HBsAg-HBsAb-HRP-HBsAg complex. TMB Substrate is used to visualize the HRP enzymatic reaction which produces a blue colored product that changes to yellow after the addition of acidic stop solution. The optical density of the developed color is read with a suitable photometer at 450nm with a selected reference wavelength at 650nm.
• Kit Components:396401A: Microtiter Strips, 1x96 wells396401B: Positive Control, 1x1ml396401C: Negative Control, 1x1ml396401D: HBsAg (HRP), 1x7ml396401E: TMB Substrate A, 1x7ml396401F: TMB Substrate B, 1x7ml396401G: Stop Solution, 1x7ml396401H: Wash Buffer, 25X, 1x25ml
• Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.