85-4775-53　［取扱停止］Es (Estrogen) BioAssay™ ELISA Kit (Bovine) 96Tests　396388

特徴

• Sample Type:Serum, plasma, tissue homogenates and other biological fluids.
• Intended Use:For quantitative detection of Es in serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity:<9.375pg/ml
• Precision:Intra-Assay CV: <8%Inter-Assay CV: <10%
• Range:15.625-1000pg/ml
• Specificity:Bovine
• Test Principle:This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Es . During the reaction, Es in the sample or standard competes with a fixed amount of Es on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Es . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Es in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Kit Components:Microtiter Strips, 1x96 wells (8x12 wells)Standard, 2x1 vialSample/Standard Dilution Buffer, 1x20mlAntibody (Biotin) (Concentrated), 1x120ulAntibody Dilution Buffer, 1x10mlStreptavidin (HRP) (SABC), 1x120ulSABC Dilution Buffer, 1x10mlTMB Substrate, 1x10mlStop Solution, 1x10mlWash Buffer, 25X, 1x30ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.