Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.
Sample Type:Cell lysates, Plasma, Serum, Urine
Intended Use:This ELISA BioAssay™ kit is used to quantitate Nitrotyrosine in samples.
Sensitivity:50nM
Range:62.5-8000nM
Specificity:Species Independent
Test Principle:This ELISA BioAssay™ Kit is a competitive assay that can be used for the quantification of Free Nitrotyrosine in plasma, serum, cell lysates, urine, and other sample matrices. The ELISA utilizes an Nitrotyrosine-coated plate and an HRP-conjugated antibody for detection which allows for an assay range of 62.5 to 8000nM Free Nitrotyrosine, with a sensitivity of 50nM. The other highlights of this BioAssay™ kit are a quick incubation time of 60 minutes, stable reagents, and an easy to use protocol.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
