Etanercept (Enbrel®) is a protein drug used to treat autoimmune diseases by adsorbing tumor necrosis factor (TNF; a soluble inflammatory cytokine)
Etanercept is a fusion protein of the extracellular ligand binding portion of TNFRSF1B and the Fc component of human immunoglobulin G1 (IgG1). It reduces the effect of naturally present TNF, functioning as a decoy receptor that binds to TNF.
Sample Type:Serum and plasma
Intended Use:The Etanercept ELISA kit is designed to measure free Etanercept with high specificity and sensitivity in biological matrices.
Sensitivity:1.5ng/ml
Recovery:250ng/mL of Etanercept was spiked in 10 lots of human serum. Recovery ranges are from 91-112% with an average recovery of 110%.
Specificity:sTNFa, TNFb, sTNFRI, sTNFRII prepared at 250ng/mL were assayed and exhibited no crossreactivity or interference.
Test Principle:This assay employs the sandwich enzyme immunoassay technique. Capture antibody is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Etanercept present in biological matrices is bound by the immobilized anti-Etanercept antibody. After washing away any unbound substances, enzyme linked anti-Etanercept antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Etanercept present in test samples. The color development is stopped and the intensity of the color is measured
Storage and Stability:Store kit components at -20°C unless specified otherwise. Unopened kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge small tubes on pulse setting prior to opening.
