Adalimumab (Trade name Humira®) is a human monoclonal antibody used to block the action of tumor necrosis factor alpha (TNF-a). TNF-a is a strong proinflammatory cytokine involved in normal immunity and also various autoimmune diseases.
Sample Type:Serum and plasma
Intended Use:The Adalimumab ELISA kit is designed to measure free Adalimumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Adalimumab molecules in biological matrices.
Sensitivity:1.5ng/ml
Specificity:hIgG1, rituximab, and infliximab prepared at 250ng/ml were assayed and exhibited no crossreactivity or interference.
Recovery:250ng/mL of Adalimumab was spiked in 10 lots of human serum. Recovery ranges are from 90-109% with an average recovery of 104%.
Test Principle:This assay employs the sandwich enzyme immunoassay technique. Anti- Adalimumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Adalimumab present in biological matrices is bound by the immobilizedcapture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Adalimumab present in test samples.
Storage and Stability:Store kit components at -20°C unless specified otherwise. Unopened kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge small tubes on pulse setting prior to opening.
