85-4774-59　［取扱停止］TPO Antibody (anti-Thyroid Peroxidase Antibody) BioAssay™ ELISA Kit (Rat) 48Tests　384852

特徴

• The TPO Antibody (anti-Thyroid Peroxidase Antibody) BioAssay™ ELISA Kit (Human) is a quantitative sandwich assay for the detection of TPO Antibody in human serum, plasma and other biological fluids.
• Sensitivity:<0.469IU/ml
• Range:0.781-50IU/ml
• Precision: Intra-Assay CV: <8%Inter-Assay CV: <10%
• Specificity:This assay has high sensitivity and excellent specificity for detection of rat TPO Antibody. No significant cross-reactivity or interference between TPO Antibody and analogues was observed.
• Assay Principle:The microtiter plate provided in this kit has been pre-coated with antigen (Thyroid Peroxidase). Standards and samples are added to the appropriate microtiter plate wells followed by biotinylated antigen (biotinylated Thyroid Peroxidase). Streptavidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. TMB substrate solution is added, after which the enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the resulting color measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TPO Antibody in the sample is then determined by comparing the O.D. of the sample to the standard curve.
• Kit Components:*384852A: Microtiter Strips, 8x12 wells
• *384852B: Standard, 2 vials384852C: Sample/Standard Dilution Buffer, 1x20ml384852D: Thyroid Peroxidase-Biotin (Concentrated), 1x120ul384852E: Antigen Dilution Buffer, 1x10ml*384852F: Streptavidin-HRP Conjugate (SABC), 1x120ul384852G: SABC Dilution Buffer, 1x10ml*384852H: TMB Substrate, 1x10ml384852J: Stop Solution, 1x10ml384852K: Wash Buffer (25X), 1x30ml
• Storage and Stability:Store unopened *384852A at 4ºC; store at -20ºC once opened. Store unopened *384852B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store *384852F and *384852H in the dark at 4°C. Store all other components at 4°C. Unused kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells
• 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C 3. Aspirate the liquid and wash plate 3 times
• 3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate the liquid and wash plate 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate the liquid and wash plate 5 times
• 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results.