Intended Use:This BioAssay™ kit is a competitive ELISA for in vitro quantitative measurement of GT in mouse serum, plasma and other biological fluids
Sensitivity:9.375pg/mL
Range:15.625-1000pg/mL
Specificity:Mouse
Test Principle:This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with mouse GT (Gastrin). During the reaction, mouse GT (Gastrin) in the sample or standard competes with a fixed amount of mouse GT (Gastrin) on the solid phase supporter for sites on the Biotinylated Detection Ab specific to mouse GT (Gastrin). Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of mouse GT (Gastrin) in the samples is then determined by comparing the OD of the samples to the standard curve.
Storage and Stability:Unused kit is stable for 6 months after receipt. Store all kit components at 4ºC. Protect HRP Conjugate and Substrate Reagent from light. For maximum recovery, centrifuge the original vials after thawing and prior to removing the cap.
