85-4742-06　TNFa, Recombinant, Mouse, aa80-235 (Tumor Necrosis Factor, DIF, TNF-alpha, TNFalpha, Tnfsf1a, TNFSF2, Cachectin) 20ug　377989

特徴

• TNFa, also known as tumor necrosis factor, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia.
• Source:Recombinant protein corresponding to aa80-235 from mouse TNFa, was expressed in E. coli.
• Molecular Weight:~17.4kD (157aa), confirmed by MALDI-TOF
• Endotoxin:<1EU/1ug (determined by LAL method)
• Biological Activity:Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is ≤0.06ng/ml.
• AA Sequence:MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.