85-4741-52　Pin1, Recombinant, Mouse, aa1-165, His-Tag (Peptidyl-prolyl Cis/Trans Isomerase NIMA-interacting 1) 100ug　377925

特徴

• Pin1, also known as peptidyl-prolyl cis-trans isomerase NIMA-interacting1, is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA, PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
• Source:Recombinant protein corresponding to aa1-165 from mouse PIN1, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~20.8kD (188aa), confirmed by MALDI-TOF
• Endotoxin:<1EU/1ug (determined by LAL method)
• Biological Activity:Specific activity is >1200nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA/minute at 37°C in Tris–HCl pH 8.0 using chymotrypsin.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSMADEEKL PPGWEKRMSR SSGRVYYFNH ITNASQWERP SGGSTVGGSS KNGQGEPAKV RCSHLLVKHS QSRRPSSWRQ EKITRSKEEA LELINGYIQK IKSGEEDFES LASQFSDCSS AKARGDLGPF SRGQMQKPFE DASFALRTGE MSGPVFTDSG IHIILRTE
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.