85-4741-50　PHPT1, Recombinant, Human, aa1-125, His-Tag (14kD Phosphohistidine Phosphatase, Phosphohistidine Phosphatase 1, Protein Janus-A Homolog, PHPT1, PHP14, CGI-202, HSPC141) 100ug　377924

特徴

• PHPT1 is a 125aa enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.
• Source:Recombinant protein corresponding to aa1-125 from human PHPT1, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~15.9kD (145aa), confirmed by MALDI-TOF
• Biological Activity:Specific activity is >120units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate/minute at pH 7.5 at 37°C.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.