85-4741-20　Ldhb, Recombinant, Mouse, aa1-334, His-Tag (L-lactate Dehydrogenase B Chain, H-Ldh, Ldh-2, LDH-B, LDH-H, Ldh2) 5ug　377883

特徴

• Ldhb, also known as L-lactate dehydrogenase B chain isoform Ldhb, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors.
• Source:Recombinant protein corresponding to aa1-334 from mouse Ldhb, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~39kD (357aa), confirmed by MALDI-TOF
• Endotoxin:<1EU/1ug (determined by LAL method)
• Biological Activity:Specific activity is >150units/mg, in which one unit will 1umole of pyruvate to L-lactate and beta-NAD/minute at pH 7.5.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSMATLKEK LIASVADDEA AVPNNKITVV GVGQVGMACA ISILGKSLAD ELALVDVLED KLKGEMMDLQ HGSLFLQTPK IVADKDYSVT ANSKIVVVTA GVRQQEGESR LNLVQRNVNV FKFIIPQIVK YSPDCTIIVV SNPVDILTYV TWKLSGLPKH RVIGSGCNLD SARFRYLMAE KLGIHPSSCH GWILGEHGDS SVAVWSGVNV AGVSLQELNP EMGTDNDSEN WKEVHKMVVD SAYEVIKLKG YTNWAIGLSV ADLIESMLKN LSRIHPVSTM VKGMYGIENE VFLSLPCILN ARGLTSVINQ KLKDDEVAQL RKSADTLWDI QKDLKDL
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.