85-4740-71　HPRT, Recombinant, Human, aa1-218, His-Tag (Hypoxanthine-guanine Phosphoribosyltransferase, HGPRT, HGPRTase, HPRT) 20ug　377837

特徴

• Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP.
• Source:Recombinant protein corresponding to aa1-218 from human HPRT1, fused to His-Tag at N- terminal, expressed in E. coli.
• Molecular Weight:~26.7kD (238aa), confirmed by MALDI-TOF
• Endotoxin:<1EU/1ug (determined by LAL method)
• Biological Activity:Specific activity is >15units/mg and is defined as the amount of enzyme that catalyze the formation of 1umole of guanosine 5’-monophosphate (GMP)/minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37°C.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.