85-4740-43　EPOR, Recombinant, Human, aa25-250, His-Tag (EPO-R, Erythropoietin Receptor) 10ug　377801

特徴

• EPOR, also known as Erythropoietin receptor, mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, it dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. So it may also activate the LYN tyrosine kinase.
• Source:Recombinant protein corresponding to aa25-250 from human EPOR, fused to His-Tag at C-terminal, expressed in insect cell.
• Molecular Weight:~25.6kD (232aa), 28-40kD (SDS-PAGE under reducing conditions.)
• Endotoxin:<1EU/1ug (determined by LAL method)
• Biological Activity:Measured by its ability to inhibit EPO dependent proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is ≤70ng/ml.
• AA Sequence:APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLDPHHHH HH
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.