85-4740-33　ECHS1, Recombinant, Human, aa28-290, His-Tag (Enoyl-CoA Hydratase, Mitochondrial, Short Chain Enoyl-CoA Hydratase, SCEH, Enoyl-CoA Hydratase 1) 20ug　377792

特徴

• Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway.
• Source:Recombinant protein corresponding to aa28-290 from human ECHS1, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~30.6kD (284aa), confirmed by MALDI-TOF
• Biological Activity:Specific activity is >150units/mg, and is defined as the amount of enzyme that hydrolyze 1umole of crotonoyl-CoA to hydroxybutyryl-CoA /minute/minute at pH 7.5 at 25°C.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.