85-4740-29　DUSP18, Recombinant, Human, aa1-188, His-Tag (Dual Specificity Phosphatase 18, DSP18, DUSP20) 20ug　377789

特徴

• Dual specificity phosphatase 18, also known as DUSP18, is a member of the dual-specificity phosphatase (DSP) family, which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues. DUSP18 is inhibited by iodoarectic acid and is activated by manganese ions. Along with having preferential enzymatic activity against phosphorylated tyrosine residues over threonine residues, DUSP18 also dephosphorylates p-nitrophenyl phosphate (pNPP) in vitro. This protein is widely expressed with highest levels in liver, brain, ovary and testis.
• Source:Recombinant protein corresponding to aa1-188 from human DUSP18, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~23.6kD (212aa), confirmed by MALDI-TOF
• Biological Activity:Specific activity is >300units/mg, and is defined as the amount of enzyme that hydrolyze 1nmole of p-nitrophenyl phosphate (pNPP)/minute at pH 7.5 at 37°C.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEV VNTLYEDIQY MQVPVADSPN SRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSG FWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.