85-4740-10　CNTF, Recombinant, Human, aa1-200, His-Tag (Ciliary Neurotrophic Factor) 100ug　377767

特徴

• CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding.
• Source:Recombinant protein corresponding to aa1-200 from human CNTF, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~25kD (220aa), confirmed by MALDI-TOF
• Biological Activity:Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is ≤1ug/ml.
• AA Sequence:MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELYQQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.