ATP1B2, as known as sodium/potassium-transporting ATPase subunit beta-2 isoform 1, is the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ ATPases. It is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The precise function of the beta-2 subunit is not known. This protein is composed of 3 subunits: alpha (catalytic), beta and gamma.
Source:Recombinant protein corresponding to aa24-188 from human ATP1B2, fused to His-Tag at C- terminal, expressed in insect cell.
Molecular Weight:~26.4kD (232aa), confirmed by MALDI-TOF
Endotoxin:<1EU/1ug (determined by LAL method)
Biological Activity:Specific activity is >3000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Adenosine 5-triphosphate to phosphate/minute/minute at pH 7.5 at 25°C
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, Baculovirus
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
