ATP1B1, also known as ATPase Na+/K+ transporting subunit beta 1, belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Recombinant protein corresponding to aa63-303 from human ATP1B1 protein, fused to His-Tag at C-terminal, expressed in insect cell.
Molecular Weight:~29kD (250aa), 28-40kD (SDS-PAGE under reducing conditions.)
Endotoxin:<1EU/1ug (determined by LAL method)
Biological Activity:Specific activity is >3000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Adenosine 5-triphosphate to phosphate/minute at pH 7.5 at 25°C.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, Baculovirus
Grade：Purified
Purity：≥90% (SDS-PAGE)
Form：Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
