Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer.
Source:Recombinant protein corresponding to the complex of human PRMT5 protein (GenBank Accession No. NM_006109), aa2-end, with N-terminal FLAG-Tag, and human MEP50 (GenBank Accession No. NM_024102), aa2-end, with N-terminal His tag, expressed in FreeStyle 293-F cells.
Molecular Weight:~73kD (PRMT5), 37.5kD (MEP50)
Assay Conditions:50ul reaction mix (20mM phosphate buffer, pH 7.4, 20uM S-adenosyl methionine, and 10-100ng methyltransferase PRMT5) add to the wells coated with the substrate. Incubate at room temperature for 1hr. Add antibody against methylated R3 residue of histone H4, incubate 1hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Specific Activity:~0.005pmole/min/ug
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ug
Source Antigen：Recombinant, FreeStyle 293-F cells
Grade：Purified
Purity：~85% (SDS-PAGE)
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 80ug/ml Flag peptide, 20% glycerol.
