CARM1 (PRMT4) methylates (mono- and asymmetric dimethylation) the arginine residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. It is recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at Arg17 (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), resulting in transcription activation via chromatin remodeling. CARM also methylates arginines in several other proteins, including EP300/P300 at Arg-2142, Arg-580, and Arg-604. CARM1 acts as coactivator for PPARG, and seems to be involved in p53/TP53 transcriptional activation.
Source:Recombinant protein corresponding to aa2-end from human PRMT4, fused to FLAG-Tag at N-terminal, expressed in in a FreeStyle 293-F cells.
Molecular Weight:~66kD
Assay Conditions:50ul reaction mix (1x HMT Assay Buffer 5, and 5-1000ng methyltransferase PRMT4) is added to wells coated with the substrate on a Thermo HBC Neutravidin black plate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ug
Source Antigen：Recombinant, FreeStyle 293-F cells
Grade：Purified
Purity：~65% (SDS-PAGE)
Form：Supplied as a liquid in 40mm Tris-HCl, pH 8.0, 110mm sodium chloride, 2.2mm potassium chloride, 80ng/ul flag peptide, 3mM DTT, 20% glycerol.
