85-4738-97　T-2 Toxin (T2) ELISA BioAssay™ Kit 96Tests　376918

特徴

• T-2 toxin is mycotoxin produced by several Fusarium species. Food crops as wheat, barely, corn, and their products are mainly contaminated. T-2 toxin is a threat for human health and animal husbandry. T-2 toxin primarily affects the blood, liver, kidneys, pancreas, muscles and function of the lymphocyte. The general symptoms of T-2 toxin poisoning are anorexia, vomiting, diarrhea, growth retardation, reproduction and neurological dysfunction. This BioAssay™ kit is a detection product developed based on ELISA technology, with operation time as short as 50 minutes.
• Sample Type:Feed 7.5ppb
• Intended Use:This BioAssay™ kit allows for the in vitro quantitative determination of T-2 toxin concentrations in feed.
• Sensitivity:0.15ppb
• Range:0.15-4.05ppb
• Precision:Intra-assay: CV%<10%Inter-assay: CV%<10%
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay™ kit has been pre-coated with T-2 toxin antigen. Standards or samples are added to the appropriate microtiter plate wells with T-2 toxin specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated T-2 toxin and T-2 toxin in standards or samples with the T-2 toxin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of T-2 toxin in the standards or samples. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 96T/48TStandard (96T), 5x1mlStandard (48T), 5x0.5mlHRP-conjugate (96T), 1x7mlHRP-conjugate (48T), 1x3.5mlAntibody (96T), 1x7mlAntibody (48T), 1x1x3.5mlTMB Substrate (96T), 1x12mlTMB Substrate (48T), 1x6mlStop Solution (96T), 1x10mlStop Solution, (48T), 1x5mlSample Diluent (2x) (96T), 1x20mlSample DIluent (2x) (48T), 1x10mlWash Buffer (10X) (96T), 1x50mlWash Buffer (10X) (48T), 1x25mlAdhesive Strip (96T/48T), 4x stripsInstruction Manual (96T/48T), 1x manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C
• Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.