85-4738-92　Sulfathiazole (ST) ELISA BioAssay™ Kit 48Tests　376916

特徴

• Sample Type:Chicken, liver, honey, milk, egg, urine, serum, pork, and other samples.
• Intended Use:This BioAssay™ kit allows for the in vitro quantitative determination of Sulfathiazole concentrations in chicken, liver, honey, milk, egg, urine, serum, pork, and other samples.
• Sensitivity:0.4ppb
• Range:0.4-32.4ppb. The standard curve concentrations used for the ELISA’s were 32.4ppb, 10.8ppb, 3.6ppb, 1.2ppb, 0.4ppb, 0ppb.
• Precision:Intra-assay: CV%<10%Inter-assay: CV%<10%
• Test Principle:This BioAssay™ employs the indirect competitive inhibition enzyme immunoassay technique. The coupling antigen is pre-coated on the micro-well stripes.( A Sulfathiazole conjugate has been pre-coated onto a microplate) Then the Sulfathiazole in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Sulfathiazole antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfathiazole in the sample. This (OD) value is compared to the standard curve and the Sulfathiazole concentration is subsequently obtained.
• Kit Components:Assay plate, 1x96TStandard, 6x1mlHRP-conjugate, 1x7mlAntibody, 1x10mlSubstrate A, 1x7mlSubstrate B, 1x7mlStop Solution, 1x7mlWash Buffer, 2x20ml (20Xconcentrate)Redissolving Solution, 3x15ml (2Xconcentrate)
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C
• Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.