85-4738-69　Malachite Green (MG) ELISA BioAssay™ Kit 96Tests　376904

特徴

• Sample Type:Fish, shrimp, water.
• Intended Use:This BioAssay™ kit allows for the in vitro quantitative determination of Malachite Green concentrations in fish, shrimp, water.
• Sensitivity:0.1ppb
• Range:0.1-27ppb
• Precision:Intra-assay: CV%<10%Inter-assay: CV%<10%
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay™ kit has been pre-coated with Malachite Green antigen. Standards or samples are added to the appropriate microtiter plate wells with Malachite Green specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Malachite Green and Malachite Green in standards or samples with the Malachite Green special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Malachite Green in the standards or samples. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate, 1x96THigh Concentration Standard (2.7ppm), 1x0.5mlHigh Concentration Negative Standard (2.5ppm), 1x0.5mlHRP-conjugate, 1x12mlAntibody, 1x6mlSubstrate A, 1x6mlSubstrate B, 1x6mlStop Solution, 1x6mlSample Diluent (5X), 1x40mlWash Buffer (10X), 1x40mlOxidant, 1x5mlAdhesive Strip (For 96 wells), 4x stripsInstruction Manual, 1x manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C
• Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.