85-4689-79　GPR161, Recombinant, Human, aa1-28, GST-Tag (G-Protein Coupled Receptor 161) 100ug　373515

特徴

• Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is roved from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown.
• Source:Recombinant protein corresponding to aa1-28 from human GPR161, fused to GST-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~30.0kD
• Amino Acid Sequence:MSLNSSLSCRKELSNLTEEEGGEGGVII
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.