85-4664-08 ALKBH, Recombinant, Human, aa1-261, GST-Tag (Alpha-ketoglutarate-dependent Dioxygenase AlkB Homolog 2) 100ug 372229
特徴
- Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
- Source:Recombinant protein corresponding to aa1-261 from human ALKBH, fused to GST-Tag at N-terminal, expressed in E. coli.
- Molecular Weight:~56.3kD
- Amino Acid Sequence:MDRFLVKGAQGGLLRKQEEQEPTGEEPAVLGGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADEIFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLSPKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKILLTKK
- Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
- Size：100ug
- Source Antigen：Recombinant, E. coli
- Grade：Purified
- Purity：≥90% (SDS-PAGE)
- Form：Supplied as a liquid in Tris, 50% glycerol.
- Molecular Weight Non Ab：56.3
- Swiss Prot Number：Q6NS38
- EU Commodity Code：30021019
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