85-4658-03　Lush, Recombinant, Drosophila Melanogaster, aa30-153, His-Tag (General Odorant-binding Protein Lush) 100ug　370680

特徴

• Odorant-binding protein required for olfactory behavior and for activity of pheromone-sensitive neurons. Binds to alcohols and mediates avoidance behavior to high concentrations of alcohols, the alcohol-binding possibly resulting in activation of receptors on T2B neurons, the activation of these receptors inhibiting these neurons. Acts in concert with Snmp and lush to capture cVA molecules on the surface of Or67d expressing olfactory dendrites and facilitate their transfer to the odorant-receptor Orco complex. Required for cVA response, probably by binding to VA. May act by serving as an adapter that bridges the presence of gaseous pheromone molecules, cVA, to activation of specific neuronal receptors expressed on T1 olfactory neurons, possibly via a specific conformational change induced by cVA that in turn activates T1 receptors. T1 neurons are excited by the pheromone VA, while T2 neurons are inhibited by alcohols. Also binds to phthalates.
• Source:Recombinant protein corresponding to aa30-153 from Drosophila melanogaster Lush, fused to His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli.
• AA Sequence:M TMEQFLTSLD MIRSGCAPKF KLKTEDLDRL RVGDFNFPPS QDLMCYTKCV SLMAGTVNKK GEFNAPKALA QLPHLVPPEM MEMSRKSVEA CRDTHKQFKE SCERVYQTAK CFSENADGQF MWP
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.