Passage 3 cells are shipped in proliferating culture with a confluence of >90%. ENDO-Growth medium containing 5% serum and growth supplement is recommended for culture. Cells have an average population doubling level of >16 when cultured.
When you receive the cells, leave the flask in 37°C CO2 incubator for 1 hour. Then, replace the transport medium with fresh ENDO-Growth medium. Let the cells grow for 24 hours before subculture.
Source:Human Saphenous Vein Endothelial Cells (HSVECs), isolated from normal human saphenous vein tissue
Cell Number:>90% confluent in T25 flask
Cell Characterization:Cytoplasmic VWF/Factor VIII: >95% positive by immunofluorescence Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence Cytoplasmic PECAM1: >95% positive by immunofluorescence
HSVECs are negative for HIV-1, HBV, HCV, and mycoplasma.
Important Note:Cells are provided either in a T-25 flask or as a 1ml frozen vial.
Store 1ml vials at -70°C. Store T-25 flasks at 37°C in 5% CO2.
