Zeocin resistant RFP-HRMECs were selected and shipped in proliferating culture with a confluence of >90% (cells are provided at passage 4-5). ENDO-Growth medium containing 5% serum and growth supplement is recommended for cell culture. Cells have an average population doubling level of >16 when cultured.
When you receive the cells, leave the flask in 37°C CO2 incubator for 1 hour. Then, replace the transport medium with fresh ENDO-Growth medium. Let the cells grow for 24 hours before subculture.
Source:RFP Expressing Human Retinal Microvascular Endothelial Cells, initiated by elutriation from dissociated normal human retinal tissue and transfected with RFP-Lentiviral particles at passage 1.
Cell Number:>90% confluent (>5x10e5cells) in T25 flask
Cell Characterization:Cytoplasmic VWF/Factor VIII: >95% positive by immunofluorescence Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence Cytoplasmic PECAM1: >95% positive by immunofluorescence
RFP-HRMECs are negative for HIV-1, HBV, HCV, and mycoplasma.
Important Note:Cells are provided either in a T-25 flask or as a 1ml frozen vial.
Store 1ml vials at -70°C. Store T-25 flasks at 37°C in 5% CO2.
