85-4653-32　［取扱停止］TNFa (Tumor Necrosis Factor Alpha) BioAssay™ ELISA Kit (Equine) 96Tests　370270

特徴

• This TNFa (Tumor Necrosis Factor Alpha) BioAssay™ ELISA Kit (Equine) is used for quantitative detection of TNFa in serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity:<9.375pg/ml
• Range:15.625-1000pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of equine TNFa. No significant cross-reactivity or interference between TNFa and analogues was observed.
• Test Principle:TNFa antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TNFa antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TNFa amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TNFa can be calculated.
• Kit Components:Micro ELISA Plate (Dismountable), 8x12Lyophilized Standard, 2xvialSample/Standard dilution buffer, 20mlBiotin-detection antibody (Concentrated), 120ulAntibody dilution buffer, 10mlHRP-Streptavidin Conjugate (SABC), 120ulSABC dilution buffer, 10mlTMB substrate, 10mlStop solution, 10mlWash buffer (25X), 30ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.