85-4644-65　［取扱停止］AP12 (Apelin 12) BioAssay™ ELISA Kit (Rat) 96Tests　369754

特徴

• Sample Type:Serum, plasma, tissue homogenates and other biological fluids.
• Intended Use:Competitive ELISA, Coated with Antigen
• Sensitivity:<0.938pg/ml
• Range:1.563-100pg/ml
• Specificity:Rat
• Test Principle:This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with AP12. During the reaction, AP12 in the sample or standard competes with a fixed amount of AP12 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to AP12. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of AP12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Kit Components:Micro ELISA Plate (Dismountable), 8x12Lyophilized Standard, 2xvialSample/Standard dilution buffer, 20mlBiotin-detection antibody (Concentrated), 60ulAntibody dilution buffer, 10mlHRP-Streptavidin Conjugate (SABC), 120ulSABC dilution buffer, 10mlTMB substrate, 10mlStop solution, 10mlWash buffer (25X), 30mlPlate Sealer, 5xpiecesProduct Description, 1xcopy
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.