85-4644-45　［取扱停止］Anti-Thyroid Peroxidase Antibody BioAssay™ ELISA Kit (Human) 96Tests　369744

特徴

• Sample Type:Serum, plasma and other biological fluids.
• Intended Use:Sandwich ELISA, Double Antigen
• Sensitivity:<1.875ng/ml
• Range:3.125-200ng/ml
• Specificity:Human
• Test Principle:This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Thyroid peroxidase was pre-coated onto 96-well plates. And the Biotin-labeled Thyroid peroxidase was used as detection antigen. The standards, test samples and Biotin-labeled Thyroid peroxidase were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the anti-Thyroid peroxidase antibody amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Thyroid peroxidase antibody can be calculated.
• Kit Components:Micro ELISA Plate (Dismountable), 8x12Lyophilized Standard, 2xvialSample/Standard dilution buffer, 20mlBiotin-detection antibody (Concentrated), 120ulAntibody dilution buffer, 10mlHRP-Streptavidin Conjugate (SABC), 120ulSABC dilution buffer, 10mlTMB substrate, 10mlStop solution, 10mlWash buffer (25X), 30mlPlate Sealer, 5xpiecesProduct Description, 1xcopy
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.