85-4644-18　［取扱停止］Alpha-Endorphin BioAssay™ ELISA Kit (Human) 48Tests　369726

特徴

• Sample Type:Serum, plasma and other biological fluids.
• Intended Use:Competitive ELISA, Coated with Antigen
• Sensitivity:<9.375pg/ml
• Range:15.625-1000pg/ml
• Specificity:Human
• Test Principle:This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with alpha-Endorphin. During the reaction, alpha-Endorphin in the sample or standard competes with a fixed amount of alpha-Endorphin on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to alpha-Endorphin. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of alpha-Endorphin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Kit Components:Micro ELISA Plate (Dismountable), 8x12Lyophilized Standard, 2xvialSample/Standard dilution buffer, 20mlBiotin-detection antibody (Concentrated), 60ulAntibody dilution buffer, 10mlHRP-Streptavidin Conjugate (SABC), 120ulSABC dilution buffer, 10mlTMB substrate, 10mlStop solution, 10mlWash buffer (25X), 30mlPlate Sealer, 5xpiecesProduct Description, 1xcopy
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.