85-4643-32　［取扱停止］Pro-endothelin 1 (Pro-ET1) BioAssay™ ELISA Kit (Human) 96Tests　369676

特徴

• The Pro-endothelin 1 (Pro-ET1) BioAssay™ ELISA Kit (Human) is a quantitative sandwich assay for the detection of Pro-ET1in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.
• Detection Range:0.871-50pg/ml
• Sensitivity:<0.469pg/ml
• Precision: Intra-Assay CV: <8%Inter-Assay CV: <10%
• Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pro-ET1. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to Pro-ET1. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pro-ET1, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pro-ET1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
• Kit Components:*369676A: Microtiter Strips, 8x12 wells
• *369676B: Standard, 2 vials369676C: Sample/Standard Dilution Buffer, 1x20ml369676D: Antibody-Biotin (Concentrated), 1x120ul369676E: Antibody Dilution Buffer, 1x10ml369676F: Streptavidin-HRP Conjugate (SABC), 1x120ul369676G: SABC Dilution Buffer, 1x10ml369676H: TMB Substrate, 1x10ml369676J: Stop Solution, 1x10ml369676K: Wash Buffer (25X), 1x30ml
• Storage and Stability:Store unopened *369676A at 4ºC; store at -20ºC once opened. Store unopened *369676B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
• Precautions for Use:1. To inspect the validity of experiment operation and the appropriateness of sample dilution, conducting a pilot experiment using standards and a small number of samples is recommended
• 2. Keep plate dry after opening and before using
• 3. Before using the kit, spin tubes and bring down all components to the bottom of tubes
• 4. Store TMB reagents in the dark
• 5. Washing process is very important. False positive results can be caused by not doing complete washes. 6. Duplicate wells are recommended for both standards and sample testing
• 7. Do not let plate go dry during the assay because this will inactivate active components
• 8. Do not reuse tips and tubes to avoid cross contamination
• 9. Avoid using the reagents from different batches together.
• Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C
• 3. Aspirate and wash plate 2 times
• 4. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times
• 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times
• 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately
• 9. Calculate results.