85-4643-18　Nocodazole Solution in DMSO, 5mg/ml 10ml　CAS No:31430-18-9　N3000-01

特徴

• Nocodazole, an inhibitor of microtubule formation, was found to greatly inhibit cell division around the mound stage as well as during the vegetative growth phase, when applied to exponentially growing Ax-2 cells. Mitotic arrest of cells with nocodazole can be used to study phosphorylation of microtubule-associated proteins which increase during mitosis, concomitant with increased microtubule dynamics. Nocodazole has been found to dramatically inhibit T cell antigen receptor (TCR) signaling, independently of its ability to depolymerize microtubules. Nocodazole arrests the cell cycle at G2/M phase and prevents phosphorylation of the T cell antigen receptor and inhibits its activity.
• Synonyms:Methyl N-(5-thenoyl-2-benzimidazolyl)carbamate; Methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate; Oncodazole; R 17934; [5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl]-carbamic acid methyl ester
• Molecular Formula:C14H11N3O3S
• Molecular Weight (dry powder):301.32
• Source:Synthetic
• Purity:Nocodazole: ≥99% (HPLC); DMSO: ≥99.9%
• Appearance:Supplied as a liquid in DMSO.
• Method for Determining Identity:Proton NMR Spectroscopic Analysis
• Concentration:5mg/ml
• Usage:Use stock solution at 5mg/ml in DMSO. Further dilutions can be made in culture media. Due to insolubility (nocodazole is only slightly soluble in water), no more than 10ug/ml concentration can be used in cell culture systems. Nocodazole is stable in biological media for seven days.
• Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at 4°C for 6 months after receipt.