85-4643-10　Uchl3, Recombinant, Mouse, aa1-230, His-Tag (Ubiquitin C-terminal Hydrolase Isozyme L3) 10ug　369152

特徴

• Uchl3, as known as ubiquitin carboxyl-terminal hydrolase isozyme L3, is a member of the peptidase C12 family of deubiquitinating enzymes. It is widely expressed with the highest levels being observed in heart, testis, thymus and striated muscle. This protein is suggested to function in the central nervous system. In particular, it is involved in the maintenance of neurons of the gracile tract, nucleus tractus, solitaris, and area postrema, and in working memory.
• Source:Recombinant protein corresponding to aa1-230 from mouse Uchl3, fused to His-Tag at C-terminal, expressed in insect cell.
• Molecular Weight:~27.2kD (238aa) 28-40kD (SDS-PAGE under reducing conditions)
• AA Sequence:MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH
• Endotoxin:~1EU/1ug (LAL)
• Specific Activity:>9000pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1pmole of ubiquitin-AMC/minute at pH 7.5, at 37°C.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.