TPST1, also known as protein-tyrosine sulfotransferase 1, is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. It utilizes 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfonate donor and binds proteins with target tyrosine residues to eventually form the tyrosine O-sulfate ester group and the desulfonated 3'-phosphoadenosine-5'-phosphate (PAP).
Source:Recombinant protein corresponding to aa26-370 from human TPST1, fused to His-Tag at C-terminal, expressed in insect cell.
Molecular Weight:~40.6kD (354aa) 40-57kD (SDS-PAGE under reducing conditions.)
Endotoxin:~1EU/1ug (LAL)
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, human from insect cell line
Grade：Highly Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
