85-4642-38　LTA, Recombinant, Human, aa35-205, His-Tag (Lymphotoxin alpha) 5ug　369094

特徴

• LTA, also known as lymphotoxin alpha and tumor necrosis factor beta, belongs to the tumor necrosis factor family. It is highly inducible, secreted, and forms heterotrimers with lymphotoxin beta which anchor lymphotoxin alpha to the cell surface. It is expressed by activated naive CD4 cells, unpolarized IL2 secreting effectors, and Th1 effectors and mediates a large variety of inflammatory, immunostimulatory, and antiviral responses.
• Source:Recombinant protein corresponding to aa35-205 from human LTA, fused to His-Tag at C-terminal, expressed in insect cell.
• Molecular Weight:~19.7kD (180aa) 18-28KD (SDS-PAGE under reducing conditions.)
• AA Sequence:ADPLPGVGLT PSAAQTARQH PKMHLAHSTL KPAAHLIGDP SKQNSLLWRA NTDRAFLQDG FSLSNNSLLV PTSGIYFVYS QVVFSGKAYS PKATSSPLYL AHEVQLFSSQ YPFHVPLLSS QKMVYPGLQE PWLHSMYHGA AFQLTQGDQL STHTDGIPHL VLSPSTVFFG AFALHHHHHH
• Endotoxin:~1EU/1ug (LAL)
• Biological activity:Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is ≤1ng/ml.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.