85-4642-33　Ldha, Recombinant, Rat, aa1-332, His-Tag (L-lactate Dehydrogenase A Chain) 10ug　369091

特徴

• Ldha, also known as lactate dehydrogenase A, catalyzes the conversion of pyruvate to lactate, utilizing NADH as a cofactor. It has been identified as a potential therapeutic target in the area of cancer metabolism. Reduction in Ldha activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.
• Source:Recombinant protein corresponding to aa1-332 from rat Ldha, fused to His-Tag at C-terminal, expressed in insect cell.
• Molecular Weight:~37.5kD (340aa) 28-40KD (SDS-PAGE under reducing conditions)
• AA Sequence:MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH
• Endotoxin:~1EU/1ug (LAL)
• Specific activity:>200units/mg, in which one unit will convert 1umole of pyruvate to L-lactate and beta-NAD/minute at pH 7.5 at 37°C.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.