85-4642-16　IL1b, Recombinant, Human, aa118-269, His-Tag (Interleukin 1 beta) 20ug　369077

特徴

• IL-1beta is an inflammatory cytokine that might modulate angiogenesis by directly interacting with vascular endothelial cells or enhancing the production of proangiogenic factors via paracrine control. IL-1beta stimulates endothelial cell migration and proliferation, adhesion-molecule expression, inflammatory mediator production, and leukocyte recruitment. IL-1beta is required for tumor growth, metastasis, and angiogenesis in several animal models.
• Source:Recombinant protein corresponding to aa118-269 from human IL1beta, fused to His-Tag expressed in E. coli.
• Molecular Weight:~21kD (189aa), confirmed by MALDI-TOF
• AA Sequence:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMVPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF LGNNSGQDII DFTMESVSS
• Endotoxin:~1EU/1ug (LAL)
• Biological Activity:Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 for this effect is ≤0.1ng/ml.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.