CTLA4, also known as cytotoxic T-lymphocyte-associated protein 4, is a co-inhibitory molecule expressed on T cells that mediates the inhibition of T-cell function. This protein is a crucial immune regulator that mediates both negative costimulation signals to T cells, and regulatory T (Treg)-cell extrinsic control of effector responses. It has been implicated in several autoimmune diseases, such as systemic lupus erythematosus.
Recombinant protein corresponding to aa36-161 from human CTLA4, fused to His-Tag at C-terminal, expressed in insect cell.
Molecular Weight:~14.6kD (135aa) 18-28KD (SDS-PAGE under reducing conditions.)
Biological Activity:Determined by the IL-2 ELISA in a using Jurkat human acute T cell leukemia cells. The ED50 range <150ng/ml with Human B7-1/CD80.
Endotoxin:<1EU/1ug (LAL)
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, human from insect cell line
Grade：Purified
Purity：≥90% (SDS-PAGE)
Form：Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
