This Cytomegalovirus purified antigen has been manufactured in response to the in-vitro diagnostics (IVD) industry’s requirement for a cost effective antigen which can demonstrate a high affinity IgG and for IgM. This Cytomegalovirus purified antigen when compared to standard lysates reduces the likelihood of cross-reactivity and minimizes lot to lot variation.
Presently, CMV infection is mostly controlled in immune compromised patients by available antiviral drugs, yet it continues to maintain its role as one of the most dangerous infectious agents for an unborn infant. Primary infection is consistently associated with the presence of a virus specific IgM antibody response. Use of this Cytomegalovirus purified antigen will aid in improving serology based assays for early detection.
Source:Human Cytomegalovirus, strain AD-169, from Human Foreskin Fibroblasts (HFF)
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:Purified virus particles were lysed in 0.2% Triton X-100 in STE buffer. The lysate was tested by passage on permissive cells for 2 weeks yielding no CPE.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
