Annexin A1 (ANXA1) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ANXA1 in human serum, plasma, tissue homogenates and other biological fluids.
Detection Range:0.312-20ng/ml
Sensitivity:0.115ng/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*023428A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use*023428B: Standard, 2x1vial023428C: Standard Diluent, 1x10ml or 1x20ml*023428D: Detection Reagent A (green), 1x60ul or 1x120ul*023428E: Detection Reagent B (red), 1x60ul or 1x120ul023428F: Assay Diluent A, 1x6ml or 1x12ml023428G: Assay Diluent B, 1x6ml or 1x12ml023428H: TMB Substrate, 1x4.5ml or 1x9ml023428J: Positive Control 2.11ng/ml, 1x1vial (Lyophilized)023428K: Stop Solution, 1x3ml or 1x6ml023428L: Wash Buffer, 30X, 1x10ml or 1x20ml
Precaution:The Stop Solution (023428K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability: Store *023428A, *023428B, *023428D, *023428E and *023428J at -20°C. After reconstitution of *023428-SP-J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once opened, it is highly recommended to use remaining reagents within 1 month.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C
3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
