This kit provides reagents for the detection of human Y-chromosome. The primers detect a region of Y chromosome in the TSPY1 gene that is not present in the X-chromosome. The amplicon is present at multiple copies within the Y chromosome which increases the sensitivity for detecting very small amount of genomic DNA and even degraded DNA.
Sample Type:All kinds of sample material suited for PCR amplification can be used. Please ensure the samples are suitable in terms of purity, concentration and DNA integrity. Always run at least one negative control with the samples. To prepare a negative control, replace the test sample with RNAse/DNAse free water.
Intended Use:When resuspended, this kit provides primers that have been tested for priming specificity, and amplification efficiency at optimal concentrations. Real-time PCR is a very sensitive technology and it is not recommended to use more or less than the specified amount of primer and probe in each reaction. However, final reaction volumes between 15ul and 50ul are often successful and maybe tested at the user’s discretion.
Sensitivity:25pg of target DNA
Negative Control:To confirm absence of contamination, a negative control reaction should be included every time the kit is used. For this reaction, the RNAse/DNAse free water should be used instead of template. A negative result indicates that the reagents have not become contaminated. If a positive result is obtained the results should be ignored and the test samples repeated. Possible sources or contamination should first be explored and removed.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
