DNA testing is the method of choice for laboratories wishing to produce fast, accurate speciation data. Primerdesign offers multiple solutions for research and applied speciation testing. All of our kits support the detection of low levels of adulterating meat. This is achieved using mitochondrial DNA as a target to enable the highest degree of sensitivity. The new Primerdesign “EASY” and “Advanced” speciation formats contain additional components to help maximize speciation accuracy.
Primerdesign’s Advanced and Easy speciation kits are supplied in a multiplex format enabling both species-specific and universal meat detection in a single well. The relative signals reported by each of these assays produces speciation percentages with minimal well usage. Furthermore, the unique application of a positive control sample as a PCR calibrator allows users to apply recognized relative qPCR calculations to increase the accuracy of their reported results.
Product features:Exceptional value for moneyRapid detection of all clinically relevant subtypesPositive copy number standard curve for quantificationHighly specific detection profileHigh priming efficiencyBroad dynamic detection range (>6 logs)Sensitive to <100 copies of targetAccurate controls to confirm findings
Intended Use:The kit includes lyophilized MasterMix as well as the DNA testing components themselves. Simply follow the easy 3 step protocol:1. Re-suspend the kit contents2. Combine the kit contents with your DNA3. Load your sample in to the genesig q16
DNA Extraction: genesig® Easy DNA extraction kits are a simple to use, low cost and powerful extraction technology suitable for virtually all sample types.
Kit Components:Target primer/probe mixLyophilized oasig MasterMixLyophilized oasig MasterMix resuspension bufferPositive control templateInternal Extraction control DNA/RNAWater50x genesig q16 reaction tubes
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
