The Mouse Kallikrein 1 (KLK1) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of KLK1 in mouse serum, plasma and other biological fluids.
Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for KLK1 has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled KLK1 and unlabeled KLK1 (standards or samples) for limited binding sites on the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of KLK1 in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of KLK1 in the sample.
Storage and Stability:Store *366234A, *366234B, *366234D and *366234E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
5. Aspirate and wash 5 times
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
