Zika virus (ZIKV) is a Flavivirus transmitted by the daytime active mosquito (Aedes aegypti). ZIKV infection during pregnancy may cause severe birth defects, and has been associated neurological disorders in adults (Guillain–Barré syndrome). An estimated 2.17 billion people are living in areas where the risk of ZIKV infection is significant.
Because of the relatively high level of conservation between the genome sequences of different flaviviruses, antibodies against the antigenic proteins of one flavivirus type frequently cross react with homologous antigens of others, rendering accurate diagnosis of specific flaviviral infections by antibody-based serological tests difficult. However, a detailed phylogenetic analysis of flavivirus sequences (including those derived from recently isolated ZIKV sequences), suggests that sufficient genetic distinctions exist in certain regions of flaviviral envelope (Env) proteins, which may be exploitable for refining the antibody capture specificities of Env-based flaviviral biomarkers.
One such region is found within the C-terminal immunoglobulin-like third domain (DIII) of the ZIKV envelope glycoprotein (ZIKV Env), a major target of antibodies elicited by flaviviruses that mediates viral entry through low-affinity interactions with glycosaminoglycans on the surfaces of host cells. ZIKV Env (DIII) is a recombinant form of DIII encoded by a ZIKV strain isolated from a patient in Brazil. It has been optimized for use as an antibody capture reagent in immunodiagnostic assays for ZIKV infection.
Recombinant protein corresponding to Zika DIII Envelope expressed in E. coli.
Applications:Suitable for use Western Blot and SDS-PAGE. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in 20mM Na-carbonate, pH 9.6. No preservative added. Reconstitute with 1ml sterile buffer or ddH2O.
